LC-MS analysis of the formed peptides from N-(O,O-diiso-propyl)phosphoryl aspartic acid |
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| Authors: | HU Jian-Jun JU Yong ZHAO Yu-FenBioorganic Phosphorus Chemistry Laboratory |
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| Affiliation: | HU,Jian-Jun JU,Yong ZHAO,Yu-FenBioorganic Phosphorus Chemistry Laboratory,Department of Chemistry,School of Life Science and Engineering,Tsinghua University,Beijing 100084,China |
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| Abstract: | LC-ESI-MS method was used to analyze the formed di-and tri-peptide hi the reaction system of N-(O,O-diisopropyl)phosphoryl aspartic acid and adenosine.Ouster ions of the peptides were given hi the ESI-MS.The structures of these small peptides were confirmed by LC-MS-MS analysis.Compared with the traditional HPLC-UV detection,this method showed good sensitivity and selectivity for peptide in the presence of compounds with strong UV absorption,such as nucle-oside and nucleotide. |
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| Keywords: | LC-ESI-MS peptide N-phosphoiyl amino acid tandem mass spectrometry cluster ion |
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