| 基于FRET机理的荧光探针的合成及其对汞离子的识别 |
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| 引用本文： | 王海菊,张文珠,何成,周硼.基于FRET机理的荧光探针的合成及其对汞离子的识别[J].发光学报,2012,33(9):1030-1036. |
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| 作者姓名： | 王海菊 张文珠 何成 周硼 |
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| 作者单位： | 大连理工大学化学学院 精细化工国家重点实验室, 辽宁 大连 116021 |
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| 基金项目： | 国家自然科学基金(21171029)资助项目 |
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| 摘 要： | 基于FRET机理设计合成了一个包含罗丹明6G及香豆素的汞离子荧光探针Rh-6G-coumarin(RC),研究了它的光谱性能及对汞离子的识别作用。在V(C2H5OH)∶V(H2O)=9∶1溶液中加入汞离子后,575 nm处荧光强度迅速增大,荧光由蓝色变为明亮的红色,同时溶液的颜色由黄色变为红色。溶液中其他金属离子,如Na+、K+、Mg2+、Fe2+、Co2+、Pb2+、Zn2+、Cd2+和Cr3+对汞离子的荧光识别没有太大影响。该探针可在较宽的pH ( 4~10)范围内识别汞离子。光谱滴定实验表明,汞离子与RC以2∶1的计量比形成了配合物。
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| 关 键 词： | 荧光探针 汞离子 FRET 罗丹明6G 香豆素 |
| 收稿时间： | 2012/6/10 |
Synthesis of Fluorescent Probe Based on FRET and Its Appalication in The Determination for Hg2+ |
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| WANG Hai-ju,ZHANG Wen-zhu,HE Cheng,ZHOU Peng.Synthesis of Fluorescent Probe Based on FRET and Its Appalication in The Determination for Hg2+[J].Chinese Journal of Luminescence,2012,33(9):1030-1036. |
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| Authors: | WANG Hai-ju ZHANG Wen-zhu HE Cheng ZHOU Peng |
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| Institution: | State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian 116021, China |
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| Abstract: | By combining rhodamine 6G and cumarin,a fluorescent probe of Hg2+,Rh-6G-cumarin(RC),based on FRET mechanism was synthesized.And through research its spectral properties and recognition to mercury ion,we found that it featured a rapid fluorescence enhancement at 575 nm upon the addition of Hg2+ in V(C2H5OH)∶V(H2O)=9∶1 solution.The fluorescence changed from blue to bright red,while the color of the solution changed from yellow to red.This probe showed high specific for Hg2+ over other metal cations,such as Na+,K+,Mg2+,Cr3+,Fe2+,Co2+,Zn2+,Cd2+ and Pb2+.It could detect Hg2+ effectively in a relatively wide pH range(4~10).The titration experiment indicated a 2∶1 stoichiometry of the RC-Hg2+ complexation species. |
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| Keywords: | fluorescent probe Hg2+ FRET rhodamine 6G cumarin |
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