| 促细胞生长包被丝素蛋白的制备 |
| |
| 引用本文： | 赵培,庞广昌,王雪青.促细胞生长包被丝素蛋白的制备[J].武汉大学学报(理学版),2011,57(4). |
| |
| 作者姓名： | 赵培 庞广昌 王雪青 |
| |
| 作者单位： | 天津商业大学生物技术与食品科学学院,天津300134／天津商业大学天津市食品生物技术重点实验室,天津300134 |
| |
| 基金项目： | 天津市科技攻关计划重点项目(06YFBZNC04200); 天津商业大学青年基金项目(090109) |
| |
| 摘 要： | 分别以聚乙二醇、明胶、海藻酸钙、壳聚糖/聚乙二醇和多聚L-赖氨五种材料作为包被物用不同方法对丝素蛋白进行预处理,制备5种丝素蛋白包被支架,并探讨它们对HeLa,293T和B16三种细胞生长的促进效果.结果表明,HeLa,293T和B16分别适合多聚L-赖氨酸、壳聚糖/聚乙二醇和海藻酸钙的丝素蛋白包被方法.在包被支架上不同细胞均呈扁平状、集落性附着在丝素蛋白纤维上.本实验获得的丝素蛋白5种包被支架增强了丝素蛋白的弹性、柔软性和黏附性,提高了丝素蛋白的可操作性,且对细胞具有较好的生物相容性和明显的促进生长作用.
|
| 关 键 词： | 丝素蛋白 包被 四唑盐(MTT)比色法 细胞活性 |
Preparation of the Coated Silk Fibroin Promoting Cell Proliferation |
| |
| ZHAO Pei,PANG Guangchang,WANG Xueqing.Preparation of the Coated Silk Fibroin Promoting Cell Proliferation[J].JOurnal of Wuhan University:Natural Science Edition,2011,57(4). |
| |
| Authors: | ZHAO Pei PANG Guangchang WANG Xueqing |
| |
| Institution: | ZHAO Pei1,2,PANG Guangchang1,WANG Xueqing1,2(1.College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China,2.Tianjin Key Laboratory of Food Biotechnology,China) |
| |
| Abstract: | Silk fibroin was coated by five materials(PEG,gelatin,calcium alginate,chitosan/PEG and poly-L-lysine) with different methods,and then five kinds of frames were prepared.Their growth-promoting effects of those frames on cells HeLa,293T and B16 were discussed.The results show that cells HeLa,293T and B16 were suitable for the coated silk fibroin by poly-L-lysine,chitosan/PEG and calcium alginate respectively.On the coated silk fibroin,different cells were flat,colony attached on silk fibroin.Those five coate... |
| |
| Keywords: | silk fibroin coated methyl thiazolyl tetrazolium(MTT) method cell activity |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
