| Study of Preparation and Characteristics of Pyridoxalated Polyhemoglobin |
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| 作者姓名： | Liang Shuguang Tong Mingrong Pan Jilun and Yu Yaoting |
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| 作者单位： | Institute for Melcular Biology，Nankai University，300071 Tianjin |
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| 摘 要： | ＳｔｕｄｙｏｆＰｒｅｐａｒａｔｉｏｎａｎｄＣｈａｒａｃｔｅｒｉｓｔｉｃｓｏｆＰｙｒｉｄｏｘａｌａｔｅｄ ＰｏｌｙｈｅｍｏｇｌｏｂｉｎＬｉａｎｇＳｈｕｇｕａｎｇ；ＴｏｎｇＭｉｎｇｒｏｎｇ；ＰａｎＪｉｌｕｎａｎｄＹｕＹａｏｔｉｎｇ（Ｉｎｓｔｉｔｕｔｅｆｏ...
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Study of Preparation and Characteristics of Pyridoxalated Polyhemoglobin |
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| Liang Shuguang, Tong Mingrong, Pan Jilun and Yu Yaoting.Study of Preparation and Characteristics of Pyridoxalated Polyhemoglobin[J].离子交换与吸附(英文版),1994(Z1). |
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| Authors: | Liang Shuguang Tong Mingrong Pan Jilun and Yu Yaoting |
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| Abstract: | Stroma--free hemoglobin (SFHb) was prepared from fresh or out--dated whole blood by lysing red cells. At different temperatures, pyridoxal 5--phosphate (PLP) was added in a 4:1 molar-ratio to the Hb tetramer followed by reduction with NaBH4 under N2 for 16 hrs subsequenl crosslinking by glutaraldehyde in presence of lysine for 14--16 hrs. Yielded soluble pyridoxalated polyhemoglobin (Poly Hb--P). 5%--20% gradient SDS--PAGE showed the change of the Hb molecular weight (MW) after crosslinking. Gel chromatography showed that the poly Hb--P had a continuous distribution of molecular weight with the range from about 65,000 to 600,000 Dalton. The poly Hb--P solution provided a normal oxygen carrying with 23.1 of P50, at pH 7.24 or 26.1 of P50 at pH 7.24 |
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| Keywords: | Blood substitute Pyridoxalated hemoglobin Pyridoxalated polyhemoglobin Chemical modification |
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