Determination of paraquat in rat brain using ion-pair solid-phase extraction and reversed-phase high-performance liquid chromatography with ultraviolet detection |
| |
| Authors: | M T Corasaniti M C Strongoli G Nisticà |
| |
| Affiliation: | FIDIA-GRF Neuropsychopharmacology Center, Girifalco, Catanzaro, Italy. |
| |
| Abstract: | ![]()
A simple and sensitive procedure for the measurement of N-methylisoquinolinium ion (NMIQ+), a putative neurotoxin, was devised using high-performance liquid chromatography (HPLC) with fluorescence detection. Separation of NMIQ+ was carried out by gel filtration and reversed-phase HPLC on a column of hydrophilic polymer gels (Asahipak GS-302H). The method was sensitive enough to measure 50 fmol of NMIQ+. Uptake of NMIQ+ into rat striatal slices was confirmed by this method. |
| |
| Keywords: | |
|
|
