Tyrosinase inhibition organic phase biosensor for triazinic and benzotriazinic pesticide analysis (part two) |
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Authors: | L Campanella R Dragone D Lelo E Martini M Tomassetti |
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Institution: | (1) Department of Chemistry, “La Sapienza” University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy |
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Abstract: | Several triazine pesticides, such as atrazine, are much more soluble in several organic solvents, such as chloroform, than
in water. Our recent research was aimed at analyzing this class of pesticides using tyrosinase OPEE (organic phase enzyme
electrodes), exploiting their inhibiting action on the tyrosinase enzyme when operating in water-saturated chloroform medium.
In this work we studied the response of a tyrosinase inhibition enzyme sensor to several triazinic (simazine, propazine, terbuthylazine)
and benzotriazinic (azinphos-ethyl and azinphos-methyl) pesticides (LOD=0.5×10−9 mol l−1). Recovery trials were also performed in vegetal matrixes (corn, barley, lentils). Lastly, the effect of the solvent (chloroform
or water) on the inhibition process was investigated via Hill’s equation and the diffusion of analyte from the solvent to
the enzyme membrane. |
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Keywords: | Triazine Benzotriazine Pesticide analysis OPEE Tyrosinase inhibition |
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