消耗型化学发光传感器的研究

本文首次发展了多种消耗型化学发光传感器, 方法基于发光试剂通过电价键全部固定在阴、阳离子交换树脂上, 在不添加外来发光试剂的情况下, 可直接对待测物进行传感。该类型传感器固定化方法简单, 灵敏度高, 还可通过控制洗脱剂的浓度精确控制发光试剂的释放量, 进而控制固定化试剂柱的使用寿命。所设计的六种类型的传感器均已成功地应用于样品中待测物的测定。

Studies of consumed chemiluminescence-based sensors

Six types of consumed chemiluminescence sensors for ascorbic acid, cyanide, Mn^2^+, Co^2^+, and H~2O~2 have been developed. It was based on the new approach that all of the reagents involved in the chemiluminescence reactions were immobilized electrostatically on Amberlyst A-27 or D151 ion-exchange resin. The analytes of interest can be sensed directly by the reaction with the chemiluminescence reagents, which were eluted by Na~3PO~4 or NaCl from the immobilization column prior to the chemiluminescence reaction. Not only these sensors have a wide linear range high sensitivity and simplicity for instrumentation, but also the immobilization methods of the chemiluminescence reagents are simple. They have been applied successfully to the determinations of analytes in various samples.