气相色谱法同时测定初烤烟叶中的4种水溶性糖

建立了一种用三甲基氯硅烷及六甲基二硅胺烷衍生化,气相色谱-氢火焰离子化检测器同时测定初烤烟叶中4种水溶性糖的检测方法。烟叶样品用50%(V/V)的乙腈溶液振荡提取,无需净化,直接用三甲基氯硅烷及六甲基二硅胺烷衍生化,产物通过微型液-液萃取净化后用气相色谱检测。通过优化前处理条件和讨论基质效应,果糖、葡萄糖、蔗糖和麦芽糖的含量均与其峰面积呈良好的线性关系(相关系数为0.9991～0.9999),添加回收率为98.14%～102.45%,重复性较好(RSD<2.2%),该方法适用于初烤烟叶中果糖、葡萄糖、蔗糖和麦芽糖的同时测定。

Determination of water-soluble sugars in curred tobacco with gas chromatography

A reliable and accurate method for the determination of four water-soluble sugars in cured tobacco by derivatization through trimethyl chlorosilane and hexamethyl disilylamine followed by gas chromatographic detection under FID mode has been established.The samples were extracted with 50%(V/V) acetonitrile by shaking,without any clean-up,the extract were directly derived by trimethyl chlorosilane and hexamethyl disilylamine.Derivative products were cleaned up by miniature liquid-liquid extraction and then detected by gas chromatography.By optimizing the pretreatment conditions and discussing the matrix effects,the method shows good linearity with correlation coefficients between 0.9991 and 0.9999,and the average recoveries were between 98.14% and 102.45% with good repeatability for fructose,glucose,sucrose and maltose,respectively.This simple,rapid and accurate method can be applied to analysis of these water-soluble sugars in cured tobacco.