| 分子印迹固相萃取-电化学发光检测牛奶中氯霉素 |
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| 引用本文： | 郝婷婷,谢文婷,李琴芬,郭智勇.分子印迹固相萃取-电化学发光检测牛奶中氯霉素[J].分析试验室,2012,31(2):105-108. |
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| 作者姓名： | 郝婷婷 谢文婷 李琴芬 郭智勇 |
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| 作者单位： | 宁波大学材料科学与化学工程学院,宁波市新型功能材料及其制备科学实验室-省部共建国家重点实验室培育基地,宁波315211 |
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| 基金项目： | 浙江省大学生科技创新活动计划(新苗人才计划)，宁波大学大学生科技创新(SRIP)项目，宁波大学学科项目 |
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| 摘 要： | 基于氯霉素(CAP)能强烈抑制Ru(bpy)32+/TPA体系的电化学发光(ECL)信号,结合分子印迹固相萃取(MISPE)样品前处理技术,建立了一种高灵敏度检测牛奶中CAP残留量的方法。在最优实验条件下,体系的ECL猝灭值ΔI与CAP浓度呈良好线性关系,线性范围为1.0×10-13～1.0×10-11g/mL,检测限为3×10-12g/mL,精密度和准确度好,可用于牛奶中CAP残留量的测定。
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| 关 键 词： | 电化学发光 分子印迹固相萃取 氯霉素 钌联吡啶 牛奶 |
A highly sensitive electrochemiluminescence method for the determination of chloramphenicol in milk combined with molecularly imprinted solid phase extraction |
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| HAO Ting-ting
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XIE Wen-ting
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LI Qin-fen
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GUO Zhi-yong.A highly sensitive electrochemiluminescence method for the determination of chloramphenicol in milk combined with molecularly imprinted solid phase extraction[J].Chinese Journal of Analysis Laboratory,2012,31(2):105-108. |
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| Authors: | HAO Ting-ting XIE Wen-ting LI Qin-fen GUO Zhi-yong |
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| Institution: | (Faculty of Materials Science and Chemical Engineering,The State Key Laboratory Base of Novel Functional Materials and Preparation Science,Ningbo University,Ningbo 315211) |
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| Abstract: | A sensitive method for the determination of chloramphenicol(CAP) in milk was developed based on its strong electrochemiluminescence(ECL) inhibition of Ru(bpy)32+/TPA system,combined with molecularly imprinted solid phase extraction(MISPE).Under the optimized conditions,the quenched ECL intensity versus the logarithm of the concentration of CAP was linear over a concentration range from 1.0 × 10-13 to 1.0 × 10-11 g/mL,and the limit of detection(LOD) was found to be 3 × 10-12 g/mL.The precision and accuracy were good.This method was successfully applied to determination of the trace CAP residue in milk. |
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| Keywords: | Electrochemiluminescence(ECL) Molecularly imprinted solid phase extraction(MISPE) Chloramphenicol(CAP) Ru(bpy)32+ Milk |
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