| 一种新型化合物的合成及对PC12高分化细胞的神经保护作用 |
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| 引用本文： | 胡爽,滕利荣,张峻榕,孟珈同,庄哲,杜梦妍,王迪,寮渭巍.一种新型化合物的合成及对PC12高分化细胞的神经保护作用[J].高等学校化学学报,2015,36(11):2322. |
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| 作者姓名： | 胡爽 滕利荣 张峻榕 孟珈同 庄哲 杜梦妍 王迪 寮渭巍 |
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| 作者单位： | 1. 吉林大学生命科学学院, 长春 1300122. 化学学院有机化学系, 长春 130012 |
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| 基金项目： | 国家自然科学基金(批准号: 81402955)资助 |
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| 摘 要： | 研究了一种新合成化合物(3h8b)的神经保护作用及初步分子机制. 该化合物具有缓解神经毒素6-羟基多巴胺(6-OHDA)和L-谷氨酸(L-Glu)诱导高分化PC12细胞损伤的作用. 可有效改善神经毒素对高分化PC12细胞的损伤, 明显增强细胞活力, 降低细胞核凋亡比率, 抑制细胞内钙离子过载. 经3h8b处理之后, 神经毒素引起的细胞内线粒体膜电位异常显著恢复. 进一步的实验表明, 3h8b可以逆转神经毒素造成的Bcl-2和Bcl-xL抑制性表达. 实验证实3h8b通过线粒体相关途经对高分化PC12细胞起保护作用, 为通过化学合成法获得具有治疗神经退行性病变作用的新型化合物提供了理论依据.
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| 关 键 词： | 神经保护 6-羟基多巴胺 L-谷氨酸 线粒体 |
Synthesis of a Novel Compound and Mediated Neuro-protection in Differentiated PC12 Cells via Mitochondria Related Pathway |
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| HU Shuang,TENG Lirong,ZHANG Junrong,MENG Jiatong,ZHUANG Zhe,DU Mengyan,WANG Di,LIAO Weiwei.Synthesis of a Novel Compound and Mediated Neuro-protection in Differentiated PC12 Cells via Mitochondria Related Pathway[J].Chemical Research In Chinese Universities,2015,36(11):2322. |
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| Authors: | HU Shuang TENG Lirong ZHANG Junrong MENG Jiatong ZHUANG Zhe DU Mengyan WANG Di LIAO Weiwei |
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| Institution: | 1. School of Life Sciences, College of Chemistry,Jilin University, Changchun 130012, China2. Department of Organic Chemistry, College of Chemistry,Jilin University, Changchun 130012, China |
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| Abstract: | The neuro-protective effect of a new synthetic compound named 3h8b on differentiated PC12(DPC12) cells against neurotoxins 6-hydroxydopamine(6-OHDA)- and L-glutamic acid(L-Glu)-induced cell injury and potential mechanisms were investigated. Data show that 3h8b significantly enhanced cell viability, improved nucleus morphological apoptotic alterations, and relieved intracellular Ca2+ overload caused by neurotoxins in DPC12 cells. The abnormal alteration of mitochondria in neurotoxins-treated cells was strongly restored by 3h8b. Further experiments demonstrate that 3h8b reverses neurotoxins-suppressed levels of B-cell lymphoma 2(Bcl-2) and Bcl-xL. All these results indicate that mitochondria related pathway is essential for the neuro-protective effect of the novel synthetic compound 3h8b against neurotoxins-induce toxicity in DPC12 cells. |
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| Keywords: | Neuroprotection 6-Hydroxydopamine L-glutamate Mitochondria |
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