| 对磺酸基杯芳烃与胃蛋白酶相互作用的光谱研究 |
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| 引用本文： | 张伟,林琳,王利丹,李永红.对磺酸基杯芳烃与胃蛋白酶相互作用的光谱研究[J].光散射学报,2010,22(1):1-5. |
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| 作者姓名： | 张伟 林琳 王利丹 李永红 |
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| 作者单位： | 郑州大学,化学系,郑州,450052 |
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| 基金项目： | 河南省基础与前沿技术研究计划项目(072300420080) |
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| 摘 要： | 模拟人体生理条件,利用荧光光谱和UV-Vis吸收光谱研究对磺酸基杯芳烃与胃蛋白酶的作用机理。由Stern-Volmer方程及UV-Vis吸收光谱得其荧光猝灭类型为静态猝灭,求出结合常数。由热力学参数判断其作用力主要为静电作用。同时根据能量转移理论得出荧光给体和受体间的距离r。并由同步荧光光谱研究得出对磺酸基杯芳烃存在下胃蛋白酶的构象发生了变化。
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| 关 键 词： | 对磺酸基杯芳烃 胃蛋白酶 荧光猝灭 |
| 收稿时间： | 2009/5/30 |
Spectroscopic Studies on the Interaction of 4-Sulfonic Calix Arene with Pepsin |
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| ZHANG Wei,LIN Lin,WANG Li-dan,LIN Yong-hong.Spectroscopic Studies on the Interaction of 4-Sulfonic Calix Arene with Pepsin[J].Chinese Journal of Light Scattering,2010,22(1):1-5. |
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| Authors: | ZHANG Wei LIN Lin WANG Li-dan LIN Yong-hong |
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| Institution: | Department of Chemistry;Zhengzhou University;Zhengzhou 450052;China |
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| Abstract: | Under physiological coditions,we investigated the interaction mechanism of 4-sulfonic calix arene with pepsin by means of fluorescence and UV-Vis absorption spectroscopics.Stern-Volmer equation and UV-Vis absorption spectroscopic indicated that the fluorescene intensity of pepsin was quenched by 4-sulfonic calix arene via a static quenching mechanism,and the associating constants were calculated.The thermodynamic parameters showed the main sort of binding force was electrostatic force.Meanwhile,the distance... |
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| Keywords: | 4-sulfonic calix arene pepsin fluorescence quenching |
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