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Determination of ethylenediaminetetraacetic acid in sea water by solid-phase extraction and high-performance liquid chromatography |
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| Authors: | Tomoko Kemmei Shuji Kodama Hironori Fujishima Atsushi Yamamoto Yoshinori Inoue Kazuichi Hayakawa |
| Affiliation: | 1. Toyama Institute of Health, 17-1 Nakataikoyama, Imizu 939-0363, Japan;2. Toyama Prefectural Environmental Science Research Center, 17-1 Nakataikoyama, Imizu 939-0363, Japan;3. Department of Biological Chemistry, College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai 487-8501, Japan;4. Products Planning & Development Department R & D Center, Nippon Filcon Co., Ltd., 2220 Ohmaru, Inagi 206-8577, Japan;5. Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan |
| Abstract: | The chelating agent EDTA is widely used, and as a result is showing up widely in the aquatic environment. Here we describe a preconcentration procedure for measuring EDTA concentration in sea water samples by HPLC. The procedure consists of forming an Fe(III) complex followed by solid-phase extraction using an activated carbon cartridge. After the preconcentration, EDTA was quantified by HPLC with ultraviolet detection (260 nm). The enrichment permitted the determination of EDTA at concentrations as low as 1 nM. Good recoveries were obtained for both brackish and full-strength sea water with high repeatability (RSD < 6%). The method was applied to sea water samples taken from near the mouth of the Oyabe River in Japan. |
| Keywords: | EDTA Solid-phase extraction Activated carbon HPLC Sea water Synthetic chelating agents |
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