Detection of protein coatings on nanoparticles surfaces by ToF‐SIMS and advanced electron microscopy |
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| Authors: | Jörg Neunzehn Felix Draude Ute Golla‐Schindler Heinrich F. Arlinghaus Hans‐Peter Wiesmann |
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| Affiliation: | 1. Max Bergmann Center of Biomaterials and Institute of Materials Science, Chair for Biomaterials, Technische Universit?t Dresden, , D‐01069 Dresden, Germany;2. Physikalisches Institut, University of Münster, , D‐48149 Münster, Germany;3. Electron Microscopy Group of Material Science, University of Ulm, , D‐89081 Ulm, Germany |
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| Abstract: | In this study, time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS) and advanced electron microscopy (scanning electron microscopy (SEM), scanning transmission electron microscopy (STEM) and transmission electron microscopy (TEM)) were applied to detect and analyse different nano‐scaled protein coatings on gold nanoparticles (NP). The NP were coated with collagen type I and fibronectin as well as different combinations of these proteins. These two main proteins in human cell organization and tissue formation were identified with the aid of ToF‐SIMS by typical amino acid mass peak detection. In addition, the protein‐coated particles were investigated by TEM and SEM to get information about the protein structure, the protein layer thickness on the particle surfaces and the reaction of NP in different protein solutions. In this study, a differentiation of diverse protein induced particle agglomeration was proven. The investigations of this study were part of the Specific Targeted Research Project CellNanoTox (project no. NMP4‐CT‐2006‐032731) funded by the European Commission under the 6th EU Framework Programme for Research and Technological Development. Copyright © 2013 John Wiley & Sons, Ltd. |
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| Keywords: | Tof‐SIMS electron microscopy nanoparticle adsorbed protein surface analytic |
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