The first fluorogenic assay for detecting a Baeyer-Villigerase activity in microbial cells |
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Authors: | Gutiérrez María C Sleegers Arthur Simpson Helen D Alphand Véronique Furstoss Roland |
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Institution: | Groupe Biocatalyse et Chimie Fine UMR-CNRS 6111, Université de la Mediterranée, Faculté des Sciences de Luminy, case 901, 163 Avenue de Luminy, 13288 Marseille, Cedex 9, France. |
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Abstract: | The first fluorogenic assay allowing for detection of microbial enzymes able to perform Baeyer-Villiger oxidation is described. This is based on the use of 4-oxopentyl umbelliferyl ether 1 as a fluorogenic substrate. When Baeyer-Villigerases active against this test ketone are present in the selected whole cells, 1 is transformed into 3-hydroxypropyl umbelliferyl ether 3, which, in a subsequent step, releases the fluorescent product umbelliferone. Different microorganisms, known to be endowed with Baeyer-Villigerase activity, were assayed. |
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