Abstract: | The structure of the N-linked oligosaccharide chain of β-momorcharin, a ribosome-inactivating protein from the seeds of Momordica charantia Linn (Cucurbitaceae) was determined. A glycopeptide liberated by pronase digestion of the glycoprotein was subjected to amino acid and neutral carbohydrate analysis to establish the composition of amino acid and sugar residues. The sequences and glycosylation linkages of the sugar and amino acid residues in the glycopeptide were determined as Manal-6(Xylβ1-2)-Manβ1-4GlcNAcβ1-4(Fucal-3)-GlcNAc-Asn-Leu by 2D-NMR spectroscopy and FAB-MS data. |