| Primary structure of p-momorcharin, a ribosome-inactivating protein from the seeds of Momordica Charantia Linn (Cucurbitaceae) |
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| 作者姓名： | 叶国杰 卢保元 金善炜 钱瑞卿 汪猷 |
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| 作者单位： | Shanghai Institute of Organic Chemistry，Chinese Academy of Sciences，Shanghai 200032，China，Shanghai Institute of Organic Chemistry，Chinese Academy of Sciences，Shanghai 200032，China，Shanghai Institute of Organic Chemistry，Chinese Academy of Sciences，Shanghai 200032，China，Shanghai Institute of Organic Chemistry，Chinese Academy of Sciences，Shanghai 200032，China，Shanghai Institute of Organic Chemistry，Chinese Academy of Sciences，Shanghai 200032，China |
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| 基金项目： | Project supported by Grants from the High Technology Development Program of China，from the National Natural Science Foundation of China(No. 29272074) |
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| 摘 要： | The complete amino acid sequence of β-momorcharin, a ribosome-inactivating protein from the seeds of Momordica charantia Linn (Cucurbitaceae) has been determined. This has been done by the sequence analysis of peptides obtained by enzymatic digestion with trypsin, chymotrypsin and S. aureus V8 protease, as well as by chemical cleavage with BNPS-skatole. The protein consists of 249 amino acid residues containing one asparagine - linked sugar group attached to the site of Asn 5 1 and has a calculated relative molecular mass of 28,452 Da without addition of the carbohydrate. Comparison of this sequence with those of trichosanthin and other ribosome-inactivating proteins from different species of plants shows a significant homology with each other. Regarding the similarity of their biological properties, an active domain of these proteins has been predicted here.
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Primary structure of p-momorcharin, a ribosome-inactivating protein from the seeds of Momordica Charantia Linn (Cucurbitaceae) |
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| YE,Guo-Jie LU,Bao-Yuan JIN,Shan-Wei
QIAN,Rui-Qing WANG,Yu.Primary structure of p-momorcharin, a ribosome-inactivating protein from the seeds of Momordica Charantia Linn (Cucurbitaceae)[J].Chinese Journal of Chemistry,1999(6). |
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| Authors: | YE Guo-Jie LU Bao-Yuan JIN Shan-Wei
QIAN Rui-Qing WANG Yu |
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| Institution: | YE,Guo-Jie LU,Bao-Yuan JIN,Shan-Wei
QIAN,Rui-Qing WANG,Yu
Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,Shanghai 200032,China |
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| Abstract: | |
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| Keywords: | Momorcharin ribosome-inactivating protein (RIP) primary structure sequence comparison |
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