Ferrocene-mediated carbon paste electrode modified with d-fructose dehydrogenase for batch mode measurement of d-fructose

A mediated modified carbon paste and renewable surface electrode for fructose amperometric measurement based on d-fructose dehydrogenase (FDH) was prepared and optimized. Commercially available ferrocene (FcH) and hydroxymethyl ferrocene (FcCH₂OH) were used as mediators. The substituted FcH showed better linearity and higher sensitivity. The influence of different experimental parameters was studied for optimum analytical performance. The final FDH-modified electrode showed good analytical performance for batch mode measurements of fructose.