基于1HNMR给药赭石后大鼠尿液的代谢组学分析 |
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引用本文: | 魏莹,万传玲,薛蓉,李晓晶,章文军,裴奉奎.基于1HNMR给药赭石后大鼠尿液的代谢组学分析[J].分析化学,2016(6):857-863. |
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作者姓名: | 魏莹 万传玲 薛蓉 李晓晶 章文军 裴奉奎 |
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作者单位: | 1. 中国科学院合成橡胶重点实验室,中国科学院长春应用化学研究所,长春 130022; 河北工业大学化工学院,天津 300130;2. 中国科学院合成橡胶重点实验室,中国科学院长春应用化学研究所,长春 130022;3. 河北工业大学化工学院,天津,300130 |
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基金项目: | 国家自然科学基金项目(Nos.20975097;21305134)资助This work was supported by the National Natural Science Foundation of China (.20975097 |
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摘 要: | 基于1H NMR 的代谢组学方法结合多变量数据分析方法(主成分分析和偏最小二乘判别分析)对灌胃给药赭石(2,5和10 g/ kg 体重剂量)的成年 Wistar 大鼠尿液进行分析,并对大鼠给药前1天、给药后1~5天尿液1 H NMR 数据进行单变量比较分析,筛选出赭石的潜在特征代谢物,对赭石引起的代谢变化进行研究,为赭石的科学用药提供依据。结果表明,大鼠体内柠檬酸、牛磺酸、肌酸酐、α-酮戊二酸、琥珀酸、二甲基甘氨酸等代谢物浓度发生明显变化,随给药时间的变化出现恢复趋势,且恢复趋势与给药剂量相关,可作为赭石的潜在特征代谢物。给药2,5和10 g/ kg 体重剂量赭石降低了大鼠机体三羧酸循环能力,影响了能量、肌酸及二甲基甘氨酸的代谢,且10 g/ kg 体重剂量赭石对大鼠肝功能造成一定影响。
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关 键 词: | 核磁共振 代谢组学 主成分分析 偏最小二乘判别分析 赭石 |
1 H NMR-Based Metabonomic Study on Urine from Haematitum-Treated Rats |
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Abstract: | Wistar rats were intragastrically administered with different doses (2, 5 and 10 g / kg body weight) of haematitum. 1H NMR-based metabonomic analysis coupled with multivariate statistical analysis (principal component analysis and partial least squares-discriminant analysis) was used to analyze the metabolic profiles of the urine samples collected from the treated rats. Univariate analysis on the 1H NMR spectra of urine (1 d before administration, 1-5 d post administration) was used to screen out the potential features of haematitum. Significant treatment related changes were observed for the levels of citrate, tuarine, creatinine,α-ketoglutarate, succinate and dimethylglycine, which could be used as potential features of haematitum. A trend of recovery in connection with dose levels was observed overtime. Such biochemical changes indicated that haematitum treatment at the dose of 2, 5 and 10 g / kg body weight affected the Krebs cycle and glucose metabolism, energy metabolism, choline metabolism and dimethylglycine metabolism in rats. These changes may attribute to the disturbances of hepatic function in 10 g / kg body weight group. |
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Keywords: | Nuclear magnetic resonance Metabonomics Principal component analysis Partial least squares-discriminant analysis Haematitum |
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