| 水相胶束体系中底物微环境对辣根过氧化物酶催化反应的影响 |
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| 引用本文： | 周勇亮,傅学奇,马林,王今堆.水相胶束体系中底物微环境对辣根过氧化物酶催化反应的影响[J].高等学校化学学报,1996,17(12):1927. |
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| 作者姓名： | 周勇亮 傅学奇 马林 王今堆 |
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| 作者单位： | 1. 吉林大学分子生物学系, 长春, 130023;
2. 吉林大学酶工程实验室, 长春, 130023 |
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| 基金项目： | 吉林大学酶工程开放实验室基金 |
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| 摘 要： | 利用荧光测活法比较了HRP在有机相与水相胶束体系中催化不同芳香胺类的动力学常数,发现在水相胶束体系中,HRP是在一个较严格的亲、疏水界面进行催化反应。同时对界面酶学性质进行了初步研究,讨论了在胶束中不同增溶位置对反应动力学常数的影响。
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| 关 键 词： | 辣根过氧化物酶 水相胶束 反应微环境 |
| 收稿时间： | 1995-12-25 |
Effect of Microenvironment of Substtates on the Catalysis of Horseradish Peroxidase in Aqueous Micelle System |
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| ZHOU Yong-Liang,FU Xue-Qi,MA Lin,WANG Jin-Dui.Effect of Microenvironment of Substtates on the Catalysis of Horseradish Peroxidase in Aqueous Micelle System[J].Chemical Research In Chinese Universities,1996,17(12):1927. |
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| Authors: | ZHOU Yong-Liang FU Xue-Qi MA Lin WANG Jin-Dui |
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| Institution: | 1. Department of Molecular Biology, Jilin University, Changchun, 130023;
2. Laboratory of Enzyme Engineering, Jilin University, Changchun, 130023 |
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| Abstract: | The kinetic constants of different aromatic amines substrates catalyzed by HRP in the organic solvent and aqueous micelle system were compared by the method of fluorescence,It was found that the catalytic reactions by HRP in the aqueous micelle system took place on a strict hydrophilic/hydrophobic interface;Meanwhile,the enzymatic properties on the interface were preliminarily studied and the effect of the substrates with different extending solvent sites in the micelle on the reaction kinetic constants was discussed. |
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| Keywords: | Horseradish perosidase Aqueous micelle Reaction microenvironment |
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