首页 | 本学科首页   官方微博 | 高级检索  
     


Optimization of Square Wave Voltammetry (SWV) Parameters by Design of Experiment (DoE) Methodologies for the Determination of Mifepristone at Glassy Carbon Electrode (GCE)
Authors:Tlou A. Makwakwa  Potlako J. Mafa  Hlengilizwe Nyoni  Titus A. M. Msagati
Affiliation:1. Department of Chemistry, College of Science, Engineering and Technology, University of South Africa, Florida, 1709 Johannesburg, South Africa

Institute for Nanotechnology and Water Sustainability, College of Science, Engineering and Technology, University of South Africa, Florida, 1709 Johannesburg, South Africa;2. Institute for Nanotechnology and Water Sustainability, College of Science, Engineering and Technology, University of South Africa, Florida, 1709 Johannesburg, South Africa

Abstract:In the present study, an electroanalytical method that permits the optimization of factors affecting SWV for sensitive detection of mifepristone at glassy carbon electrode (GCE) using response surface methodology (RSM) with desirability function (DF) is presented. Factors selected for optimization after screening using full factorial design were frequency (X1), amplitude (X2), and pH (X3). The central composite design as a response surface methodology with desirability function (DF) was applied for obtaining the optimum level. The optimum conditions were obtained as follows: Frequency (X1=50 Hz), amplitude (X2=33.4 mV), and pH (X3=9.4), with an overall desirability function of 0.97. Subsequently, confirmatory experiments were performed in triplicates to validate the optimum conditions. The results obtained were satisfactory and agreed well with less only 11.9 % deviation from the values predicted by the model. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.54 and 1.80 ppm, respectively. The proposed method was applied for a quantitative determination of mifepristone in spiked tap water samples. The recovery tests showed that the detection of mifepristone at GC could be evaluated on environmental samples.
Keywords:Mifepristone  square wave voltammetry  Glassy carbon electrode  Design of experiment  Aquatic environment
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号