Institution: | 1. Van Yuzuncu Yil University, Faculty of Science, Department of Analytical Chemistry, 65080 Van, Turkey
Aynda Private Technical Institute, Department of Pharmacy, 44001 Erbil, Iraq;2. Van Yuzuncu Yil University, Faculty of Science, Department of Analytical Chemistry, 65080 Van, Turkey
Knowledge University, College of Science, Department of Medical Laboratory Science, Kirkuk, 44001 Erbil, Iraq;3. Van Yuzuncu Yil University, Faculty of Pharmacy, Department of Analytical Chemistry, 65080 Van, Turkey |
Abstract: | An effective electrochemical sensor was constructed using an unmodified boron-doped diamond electrode for determination of genistein by square-wave voltammetry. Cyclic voltammetric investigations of genistein with HClO4 solution indicated that irreversible behavior, adsorption-controlled and well-defined two oxidation peaks at about +0.92 (PA1) & +1.27 V (PA2). pH, as well as supporting electrolytes, are important in genistein oxidations. Quantification analyses of genistein were conducted using its two oxidation peaks. Using optimized experiments as well as instrumental conditions, the current response with genistein was proportionately linear in the concentrations range of 0.1 to 50.0 μg mL?1 (3.7×10?7?1.9×10?4 mol L?1), by the detection limit of 0.023 μg mL?1 (8.5×10?8 mol L?1) for PA1 and 0.028 μg mL?1 (1.1×10?7 mol L?1) for PA2 in 0.1 mol L?1 HClO4 solution (in the open circuit condition at 30 s accumulation time). Ultimately, the developed method was effectively applied to detect genistein in model human urine samples by using its second oxidation peak (PA2). |