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Antibody-invertase Cross-linkage Nanoparticles: A New Signal Tag for Point-of-Care Immunoassay of Alpha-fetoprotein for Hepatocellular Carcinoma with Personal Glucometer |
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| Authors: | Ling Li Dong Liang Wuhua Guo Dianping Tang Yongyi Zeng |
| Institution: | 1. The First Clinical Medical College of Fujian Medical University, Fuzhou, 350004 P. R. China
Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025 P. R. China Hepatopancreatobiliary Surgery Department, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350004 P. R. China Ling Li and Dong Liang contributed equally to this work.;2. Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025 P. R. China People's Hospital Affiliated of Fujian University of Traditional Chinese Medicine, Fuzhou, 350004 P. R. China Ling Li and Dong Liang contributed equally to this work.;3. Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025 P. R. China;4. Department of Chemistry, Fuzhou University, Fuzhou, 350108 P. R. China;5. The First Clinical Medical College of Fujian Medical University, Fuzhou, 350004 P. R. China |
| Abstract: | Methods based on immunoassays have been developed for cancer biomarker alpha-fetoprotein (AFP), but most involve complicated or stripping procedures and are unfavourable for routine use. Herein we report the proof-of-concept of simple and point-of-care (POC) immunoassay for AFP in hepatocellular carcinoma on a portable personal glucometer (PGM) by using antibody-invertase cross-linkage nanoparticles as the signal-generation tags. Antibody-invertase cross-linkage nano tags were synthesized by using reverse micellar method with glutaraldehyde. The POC immunoassay was carried out on monoclonal anti-AFP capture antibody-coated microplate with a sandwich-type reaction mode. Introduction of invertase nano labels accompanying target AFP could hydrolyse sucrose into glucose and fructose. The produced glucose molecules could be determined on a portable personal glucometer. Relative to unimolecular invertase labelling, improved analytical features were acquired with antibody-invertase nano labelling. With the nano labels, PGM-based immunoassay exhibited good electrochemical responses for the detection of AFP, and allowed detection of AFP at a concentration as low as 5.4 pg mL?1. Moreover, a good repeatability and intermediate precision could be found down to 12.68 %. Good well-matched results were obtained for analysis of human serum specimens between POC immunoassay and commercial human AFP ELISA kit. |
| Keywords: | Electrochemical immunoassay point-of-care testing alpha-fetoprotein personal glucometer antibody-invertase nanoparticles |