钯(II)与阿莫西林和氨苄西林相互作用的共振瑞利散射光谱及其分析应用

在酸性介质中加热, 使阿莫西林(AMO)和氨苄西林(AMP)等侧链含苄氨基的青霉素类抗生素发生降解, 其降解产物青霉胺和苄氨基青霉醛在pH 5左右的弱酸性介质中能进一步与钯(II)反应形成物质的量比为1∶1∶1的混配型三元配合物, 此时将引起共振瑞利散射(RRS)的显著增强, 并出现新的RRS光谱. 钯(II)与两种药物的反应产物具有相似的RRS光谱特征, 最大散射波长均位于370 nm. 在一定范围内散射增强(ΔI)与药物的浓度成正比. 该方法具有较高的灵敏度, 对于AMO和AMP的检出限(3δ)分别为18.0和15.4 ng•mL^－1. 此时侧链不含苄氨基的其他青霉素不产生类似反应, 并且也允许一定量的其它物质存在, 因此, 方法有较好的选择性, 可用于胶囊、片剂及血清、尿样中阿莫西林和氨苄西林的测定, 能获得较满意的结果.

Resonance Rayleigh Scattering Spectra of Interaction of Amoxicillin or Ampicillin with Palladium(II) and Their Analytical Applications

Heated in acidic medium, penicillin antibiotics containing an amino group side chain such as amoxicillin (AMO) and ampicilliin (AMP) can be degraded, and the penicillamine and benzyl aminopeniloaldehyde of the degradation products can further react with palladium(II) to form a composition ratio 1∶1∶1 blended ternary complex, which leads to the great enhancement of resonance Rayleigh scattering (RRS) and appearance of new RRS spectra. The spectral characteristics of the reaction products of the two drugs with palladium(II) are similar, and the maximum wavelengths are all at 370 nm. The scattering intensity increments (ΔI) of the products are directly proportional to the concentrations of the antibiotics in certain ranges. The method exhibits higher sensitivity, and the detection limits (3δ) for AMO and AMP are 18.0 and 15.4 ng• mL^－1, respectively. In this case, the other penicillin antibiotics without any amino group in the structure of the side chain have not similar reactions, and certain amounts of some coexisting substances have no interference. Hence, the method has a good selectivity. It can be applied to the determination of amoxicillin and ampicillin in capsule, tablet, human serum and urine samples with satisfactory results.