薄层色谱与表面增强拉曼散射光谱联用技术的研究进展

表面增强拉曼光谱（SERS）作为一种快速、灵敏的分析技术，被广泛应用于分析化学、环境检测及食品安全等领域。在实际生活中的样品大多为混合物，直接使用SERS技术无法对复杂样品中的分析物进行准确测定。薄层色谱（TLC）分离技术具有操作简便，成本低廉及分离速度快等特点，TLC作为一种高通量的分离技术在合成化学、分析化学、药物化学及食品科学等研究领域得到了广泛的应用。TLC对待测物体系进行分离后，通过碘显色或荧光对分离的斑点进行可视化处理，再结合质谱，红外光谱、荧光光谱及SERS光谱等分析技术可以对分离物质进行定性及定量分析。TLC与SERS联用技术的出现，使得SERS光谱可以应用于混合物中分析物的有效测定。TLC-SERS技术同时具备良好的分离作用和灵敏的光谱检测性能，适用于对复杂样品进行分离检测。在TLC-SERS检测过程中，样品用量少且无需使用复杂的实验设备即可实现对混合物现场快速检测。介绍了SERS的增强机理以及活性基底的制备，对TLC-SERS技术在环境污染物检测、食品安全、中草药鉴定及生物医学等方面的应用做了概括性综述。给出了TLC-SERS技术在有害物快检领域的应用实例，为TLC-SERS技术未来用于食品安全、法医鉴定及环境治理中快速检测方法建立及仪器设备研发提供参考。

Research Progress of Thin Layer Chromatography and Surface Enhanced Raman Scattering Spectroscopy

Surface Enhanced Raman spectroscopy(SERS)is a sensitive,instant and nondestructive sensing technique that has been widely used in analytical chemistry,environmental protection and food safety.However,the samples in real-world usually contain many different components,which makes the identification of analytes from mixed samples very difficult for SERS sensing.For their advanced features such as simple operation process,cost-effective and instant separation time,thin layer chromatography(TLC)technology was widely employed in synthetic chemistry,analytical chemistry,medicinal chemistry and food science as a powerful separation strategy.The target system was firstly separated by TLC,and the corresponding spots of analyte are visualized by iodine colorimetry or fluorescence,and then the analytes werequalitative and quantitative identified by mass spectrometry,FTIR,fluorescence spectroscopy and SERS.The in-tandem of SERS with thin layer chromatography(TLC-SERS)is very promising in effectively sensing analytes from mixture samples due to its high throughput and sensitivity and the separation ability.The complicated apparatuses are not necessary during the application process of TLC-SERS.This paper introduced the mechanism of SERS enhancement and the preparation of SERS substrates,and this review highlights recent progress of TLC-SERS in the view from the application,such as in monitoring pollutants in the environment,food safety,identification of herbal medicine and biomedicine.The application example of TLC-SERS method on instant inspection of harmful ingredients was then presented,which provides a reference for future instant inspection method construction and development of rapid detection instruments in the area of food safety,forensic characterization and environmental protection.