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Electroanalysis of kaempferol using pyrolytic graphite and a hemoglobin/polysorbate-20 modified electrodes
Authors:X F Zheng  J Zao  G F Chen  S L Liu  G X Li
Institution:(1) Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210093, China;(2) School of Life Science and Shanghai Key Laboratory of Bioenergy Crops, Shanghai University, Shanghai, 200444, China
Abstract:The electrochemical behavior of kaempferol, one of the most common flavonoids, was studied using a pyrolytic graphite electrode and a hemoglobin/polysorbate-20 modified electrode. The modified electrode was found to be applicable for kaempferol determination. The linear calibration was in the range from 4 × 10−7 to 4 × 10−5 M, with a limit of detection (LOD) of 1 × 10−7 M. The relative standard deviation (RSD) was 3.3% for ten successive determinations. The proposed method is sensitive, has a high reproducibility, and a wide detection range. The text was submitted by the authors in English.
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