Organic Profiles and Pattern Recognition Analysis for Clinical Applications |
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Authors: | Kyoung Rae Kim |
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Institution: | College of Pharmacy, Sungkyunkkwan University, Suwon 440-746, Korea |
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Abstract: | Efficient gas chromatographic (GC) and capillary electrophoretic (CE) profiling methods were combined with simple pattern recognition methods for the correlation between urinary organic profiles (organic acids,polyamines and nucleosides) and uterine cervical cancer.For the urinary organic acid profiles, free organic acids were recovered by dual solid-phase extraction (SPE) in anion-exchange and normal-phase partition modes after methoximation of keto acids in alkalinized urine samples, followed by conversion to tert.-butyldimethylsilyl derivatives for the direct analysis by dual-capillary column GC. |
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