Determination of Paeoniflorin in Total Glucosides of Peony Root by HPLC

The crude drug of Radix Paeoniae Alba has been used extensively in traditional Chinese medicine as an analgesic, an antispasmodic,an astringent, and a sedative for the treatment of a variety of painful afflictions. A modern way of using Radix Paeoniae Alba is the application of Total Glucosides of Peony Root (TGPR), which is the ethanol extract from the crude drug. Our interest in this work is to develop a method to determine one of the main effective components, Paeoniflorin, in TGPR by HPLC. Although several HPLC methods for the determination of Paeoniflorin were reported before,our method has the advantages of simplicity, rapidity and accuracy.