紫外-可见光谱法研究二茂铁衍生物与血红素的相互作用

采用紫外-可见光谱法(UV-Vis)研究三种二茂铁衍生物Fc(COOH)₂(λ_max=286 nm), Fc(OBt)₂(λ_max=305 nm), Fc(Cys)(λ_max=289 nm)]与血红素(heme, λ_max=386 nm)的相互作用。实验结果表明：当固定heme浓度时，heme的吸光度随着Fc(COOH)₂和Fc(Cys)浓度的增加而增大，而heme的吸光度随着Fc(OBt)₂的浓度的增加几乎没有增大;当分别固定Fc(COOH)₂, Fc(Cys)和Fc(OBt)₂的浓度时，Fc(COOH)₂和Fc(Cys)的吸光度随着heme浓度的增加而增大，而Fc(OBt)₂的吸光度随着heme浓度的增加没有变化，说明Fc(COOH)₂和Fc(Cys)与heme存在分子间的相互作用，主要是由于Fc(COOH)₂和 Fc(Cys)与heme能形成氢键，分子链增长，吸收的能量增加，导致吸光度增大;而Fc(OBt)₂与heme没有分子间的相互作用，是由于Fc(OBt)₂没有自由的氢，不能与heme形成分子间的氢键。同时考察了三种二茂铁衍生物与heme 的吸光度随时间的变化，Fc(COOH)₂和 Fc(Cys)与heme的吸光度随着时间的增加而减少，而Fc(OBt)₂与heme的吸光度随时间的变化几乎没有变化。Fc(COOH)₂与Fc(Cys)和heme的反应时间为0.5，18和48 h，当固定Fc(COOH)₂浓度时，在λ_max=384 nm处的吸光度由2.64分别变为2.53和2.51;当固定heme的浓度时，在λ_max=384 nm处的吸光度由1.76分别变为1.72和1.68;当固定Fc(Cys)浓度时，在λ_max=397 nm处的吸光度由2.74分别变为2.63和2.55;当固定heme的浓度时，在λ_max=397 nm处的吸光度由1.82分别变为1.58和1.49。

Interaction Study of Ferrocene Derivatives and Heme by UV-Vis Spectroscopy

The interaction between ferrocene derivatives,such as Fc(COOH)2 (λmax=286 nm),Fc(OBt)2 (λmax=305 nm),Fc(Cys)(λmax=289 nm)and heme(λmax=386 nm)were studied by UV-Vis spectroscopy,respec-tively.The results show that,when the concentration of heme is fixed,the absorbance of heme increases with the increase of Fc(COOH)2 and Fc(Cys)concentration,the absorbance of heme almost keep the same when Fc(OBt)2 concentration increases;when the concentration of ferrocene derivatives are fixed,the absorbance of Fc(COOH)2 and Fc(Cys)also increases with the increase of heme concentration,the absorbance of Fc(OBt)2 almost keep the same when heme concentration increase.It is demonstrated that the hydrogen bonding interac-tions happen between Fc(COOH)2 ,Fc(Cys)and heme,none of Fc(OBt)2 ,the formation of hydrogen bonding lead to the growth of molecular chain,the bigger molecule can absorb more energy and increase the absor-bance.Meanwhile,the stability of molecule is affected by the formation of hydrogen bonding,when the reac-tion time increases from 0.5 h to 18 h and 48 h,the absorbance atλmax=384 nm change from 2.64 to 2.53 and 2.51 with fixed concentration of Fc(COOH)2 ,the absorbance atλmax=384 nm change from 1.76 to 1.72 and 1.68 with fixed concentration of heme,the absorbance atλmax=397 nm change from 2.74 to 2.63 and 2.55 with fixed concentration of Fc(Cys),and the absorbance atλmax=397 nm change from 1.82 to 1.58 and 1.49 with fixed concentration of heme,respectively.