Extraction and analysis of ellagic acid from novel complex sources |
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| Authors: | Antonio F Aguilera-Carbo Christopher Augur Lilia A Prado-Barragan Cristóbal N Aguilar Ernesto Favela-Torres |
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| Institution: | 1.Department of Biotechnology,Universidad Autónoma Metropolitana,Iztapalapa,DF, México;2.IRD-Unité Biotrans, IMEP Case 441, Faculté des Sciences de Saint Jér?me,Université Paul Cézanne,Marseille cedex 20,France;3.Food Research Department, School of Chemistry,Universidad Autónoma de Coahuila,Saltillo, Coahuila,México |
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| Abstract: | Ellagic acid (EA) was quantified by reversed-phase high-performance liquid chromatography (RPHPLC) coupled with photodiode
array detection (DAD) in five fine-powdered plants collected from the semiarid region of Mexico. Samples analysed included
Jatropha dioica branches (Dragon’s blood), Euphorbia antisyphyllitica branches (Candelilla), Turnera diffusa Willd leaves (Damiana), Flourensia cernua leaves (hojasén) and Punica granatum husk (pomegranate) at two maturity stages (“turning” or intermediate and maturated fruit, considered as positive controls).
The results demonstrated high EA concentrations in all tested samples which are novel sources of this natural antioxidant.
The method developed for the EA analysis is fast and it showed an excellent linearity range, repeatability, intra-and inter-day
precision and accuracy with respect to the methods reported for the EA analysis. |
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| Keywords: | ellagic acid semiarid plants HPLC analysis |
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