Comparative proteomic analysis of grain development in two spring wheat varieties under drought stress |
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Authors: | P?Ge C?Ma S?Wang L?Gao X?Li G?Guo Email author" target="_blank">W?MaEmail author Email author" target="_blank">Y?YanEmail author |
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Institution: | (1) College of Life Sciences, Capital Normal University, Beijing, 100048, China;(2) Centre for Comparative Genomics, Murdoch University, Perth, Western Australia, 6150, Australia;(3) Present address: Western Australia Department of Agriculture & Food, Perth, Western Australia, 6150, Australia; |
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Abstract: | Two spring wheat varieties Ningchun 4 and Chinese Spring with good and poor resistance to abiotic stress, respectively, were
used to investigate proteomic changes in the developing grains under drought stress by a comparative proteomics approach.
A total of 152 protein spots showed at least twofold differences in abundance on two-dimensional electrophoresis (2-DE) maps,
of which 28 and 68 protein spots were identified by MALDI-TOF and MALDI-TOF/TOF mass spectrometry, respectively. Of the 96
identified protein spots, six different expression patterns were found and they were involved in stress/defense/detoxification,
carbohydrate metabolism, photosynthesis, nitrogen metabolism, storage proteins and some other important functions. Comparative
proteomic analysis revealed that under the drought conditions the decreased degree of ascorbate peroxidases was more significant
in Chinese Spring than in Ningchun 4 during grain development whereas translationally controlled tumor protein, which was
significantly upregulated at 14 DAF, was present in Ningchun 4 and absent in Chinese Spring. The Rubisco large subunit displayed
an upregulated expression pattern in Ningchun 4. In addition, two drought-tolerant proteins, triosephosphate isomerase and
oxygen-evolving complex showed B and F type expression patterns in Chinese Spring, but D and B types in Ningchun 4, respectively.
These differentially expressed proteins might be responsible for the stronger drought resistance of Ningchun 4 compared to
Chinese Spring. |
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