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Single Chlamydomonas reinhardtii cell separation from bacterial cells and auto-fluorescence tracking with a nanosieve device |
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| Authors: | Grant Korensky Xinye Chen Mengdi Bao Abbi Miller Blanca Lapizco-Encinas Myeongkee Park Ke Du |
| Institution: | 1. Department of Mechanical Engineering, Rochester Institute of Technology, Rochester, NY, USA
Both authors have contributed equally to this work.;2. Department of Mechanical Engineering, Rochester Institute of Technology, Rochester, NY, USA Department of Microsystems Engineering, Rochester Institute of Technology, Rochester, NY, USA Both authors have contributed equally to this work.;3. Department of Mechanical Engineering, Rochester Institute of Technology, Rochester, NY, USA;4. Department of Biomedical Engineering, Rochester Institute of Technology, Rochester, NY, USA;5. Department of Chemistry, Dong-A University, Busan, Republic of Korea |
| Abstract: | A planar, transparent, and adaptable nanosieve device is developed for efficient microalgae/bacteria separation. In the proposed method, a sacrificial layer is applied with dual photolithography patterning to achieve a 1D channel with a very low aspect ratio (1:10 000). A microalgae/bacteria mixture is then introduced into the deformable PDMS nanochannel. The hydrodynamic deformation of the nanochannel is regulated to allow the bacteria cells to pass through while leaving the microalgae cells trapped in the device. At a flow rate of 4 μL/min, the supernatant collected from the device is indistinguishable from a control solution, indicating that nearly all the microalgae cells are trapped in the device. Additionally, this device is capable of single cell auto-fluorescence tracking. These microalgae cells demonstrate minimal photobleaching over 250 s laser exposure and could be used to monitor hazardous compounds in the sample with a continuous flow. This method will be valuable to purify microalgae samples containing contaminations and study single-cell heterogeneity. |
| Keywords: | Bacteria Fluorescence Microalgae Microfluidics Separation |