Bioamperometric Systems with Fructose Dehydrogenase From Gluconobacter japonicus for D-Tagatose Monitoring |
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| Authors: | Edita Voitechovič Jonita Stankevičiūtė Aušra Vektarienė Gytis Vektaris Regina Jančienė Nomeda Kuisienė Julija Razumienė Rolandas Meškys |
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| Institution: | 1. Institute of Biochemistry, Life Sciences Center, Vilnius University, Saulėtekio av.7, LT-10257 Vilnius, Lithuania;2. Institute of Theoretical Physics and Astronomy, Vilnius University, Saulėtekio av. 3, LT-10222 Vilnius, Lithuania;3. Institute of Biosciences, Life Sciences Center, Vilnius University, Saulėtekio av.7, LT-10257 Vilnius, Lithuania |
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| Abstract: | D-Tagatose is a rare natural sugar, which is promising alternative for popular dietary sweeteners. To optimize and make D-tagatose production more cost-effective, a portable (bio)sensor would be beneficial for D-tagatose quantification in bioreactor media during D-tagatose (bio)synthesis. In this study, a pilot study of D-tagatose quantification by a bioamperometric system is reported. A key feature of the system is usage of fructose dehydrogenase from Gluconobacter japonicus, widely known as a highly fructose-specific enzyme, and 2-arylamino-1,4-benzoquinone derivatives, as electron transfer mediators from the enzyme to carbon electrodes. The system has been validated as biosensor for D-tagatose quantification in the fermentation medium. |
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| Keywords: | D-tagatose bioelectrocatalysis fructose dehydrogenase quantum chemical analysis carbon electrode |
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