Fabrication of Electrochemical Immunosensor for Detection of Interleukin 8 Biomarker via Layer-by-layer Self-assembly Process on Cost-effective Fluorine Tin Oxide Electrode |
| |
Authors: | Elif Burcu Aydın Mustafa Kemal Sezgintürk |
| |
Institution: | 1. Tekirdağ Namık Kemal University, Scientific and Technological Research Center, Tekirdağ, Turkey;2. Çanakkale Onsekiz Mart University, Faculty of Engineering, Bioengineering Department, Çanakkale, Turkey |
| |
Abstract: | A highly sensitive impedimetric immunosensor for the determination of interleukin 8 (IL 8) cancer biomarker was fabricated via simple fabrication process. A silane agent with isocyanate groups, 3-(triethoxysilyl)propyl isocyanate (IPTES), were utilized for binding of IL 8 antigen specific antibodies and self-assembled on the fluorine tin oxide (FTO) substrate with a standard silanization chemistry. Under optimum experimental conditions, the designed immunosensor had a wide linear detection range of 0.02–4 pg/mL, a low limit of detection of 11.9 fg/mL and a high sensitivity of 4.42 kΩ pg−1 mL cm−2. The designed biosensor displayed high reproducibility and repeatability, good selectivity against interference biomarkers. |
| |
Keywords: | Interleukin 8 Fluorine tin oxide 3-(triethoxysilyl)propyl isocyanate Disposable electrode |
|
|