| Institution: | 1. CSIR – National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi, 110012 India
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002 India;2. CSIR – National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi, 110012 India;3. Department of Chemistry, Hans Raj College, University of Delhi, Delhi, 110007 India;4. Laboratory of Bio-Physio Sensors and Nanobioengineering, School of Biochemical Engineering, Indian Institute of Technology (BHU), Varanasi, 221005 Uttar Pradesh, India |
| Abstract: | Folic acid (FA) deficiency is associated with several clinical conditions such as megaloblastic anemia, neuropsychiatric, and pregnancy-related syndromes, this makes FA an important metabolite to be monitored. We have fabricated an electrochemical biosensor based on gold nanoparticles decorated molybdenum disulfide nanosheets (AuNPs−MoS2NSs) nanocomposite as a transducer matrix for specific and rapid electrochemical detection of FA. Differential pulse voltammetry (DPV) studies displayed a rapid analytical response of the fabricated AuNPs−MoS2NSs/GCE sensor probe towards FA in a wide concentration range of 0.001–100 μM with a very low detection limit of 0.72±0.03 nM. The selectivity of the fabricated sensor probe has been examined in the presence of interferents such as dopamine, uric acid, ascorbic acid, glucose, and urea. The clinical potential of the fabricated biosensor was established by monitoring FA in human serum samples. The developed AuNPs−MoS2NSs/GCE sensor probe showed high reproducibility and stability, indicating its promise for FA detection in clinical settings. |
