| 小型可连续进样微流控芯片分析仪的研制 |
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| 引用本文： | 徐章润,王世立,樊晓峰,王福仁,方肇伦.小型可连续进样微流控芯片分析仪的研制[J].分析化学,2003,31(12):1527-1530. |
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| 作者姓名： | 徐章润 王世立 樊晓峰 王福仁 方肇伦 |
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| 作者单位： | 东北大学分析科学研究中心 沈阳110004
(徐章润，王世立，樊晓峰，王福仁)，东北大学分析科学研究中心 沈阳110004(方肇伦) |
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| 基金项目： | 国家自然科学基金资助项目 (No .2 9870 0 1) |
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| 摘 要： | 报道了一种结构简单、可连续进样的小型微流控芯片分析仪的研制。顺序注射分析系统通过芯片上制作的接口将试样连续引入芯片 ,并采用自行设计的紧凑型光纤式激光诱导荧光检测器进行检测。该仪器用于芯片毛细管电泳分离实验室合成Cy5荧光染料 ,实现了连续进样和换样。峰高RSD为 1 .9% (n=1 1 ) ,试样通量 3 5 h ;相邻试样携出 <4%。
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| 关 键 词： | 小型可连续进样微流控芯片分析仪 微流控分析系统 光纤式激光诱导荧光检测器 顺序注射分析技术 |
The Development of a Compact Microfluidic Chip Analyzer with Continuous Sample Introduction |
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| Xu Zhangrun,Wang Shili,Fan Xiaofeng,Wang Furen,Fang Zhaolun.The Development of a Compact Microfluidic Chip Analyzer with Continuous Sample Introduction[J].Chinese Journal of Analytical Chemistry,2003,31(12):1527-1530. |
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| Authors: | Xu Zhangrun Wang Shili Fan Xiaofeng Wang Furen Fang Zhaolun |
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| Institution: | Xu Zhangrun,Wang Shili,Fan Xiaofeng,Wang Furen,Fang Zhaolun * |
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| Abstract: | A novel microfluidic chip analyzer was developed, in which the capillary electrophoresis (CE) separation with laser induced fluorescence (LIF) detection, simplified optics involving the use of optical fibers, capable of non-interrupted continuous sample introduction using a sequential injection (SI) system were used. The performance of the system is demonstrated in the separation of fluorochrome Cy5 from impurities. Low carryover (<4%) among samples was achieved at a sampling frequency of 35/h with a precision of 1.9% RSD ( n 11). |
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| Keywords: | World-to-chip interfacing microfluidic chips capillary electrophoresis laser induced fluorescence sequential injection |
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