Adsorption of CETP on monolayers formed from HDL extracted lipids |
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| Institution: | 1. Laboratoire de Biochimie et de Biologie moléculaire, CHU Angers, Angers, France;2. Biophysique Pharmaceutique, UPRES EA 2169, Vectorisation particulaire, Université d’Angers, Angers, France;1. Bi Biophotonics Co., Ltd., Gimpo-si, Kyunggi-do 10090, South Korea;2. Department of Pharmacy, College of Pharmacy, Kyung Hee University, Seoul 02447, Republic of Korea;3. Department of Chemistry, Seoul Women’s University, Seoul 01797, South Korea;1. State Key Laboratory of Bioelectronics & National Demonstration Centre for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China;2. Anhui Huaneng Cable Group Co., LTD Bawan Industrial Zone, Gaogou Town, Wuwei City, Wuhu 341400, PR China;3. School of Chemistry and Life Science, Suzhou University of Science and Technology, Suzhou 215009, PR China;4. School of Life Science, Nantong University, 226019, PR China;1. Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, Shandong, China;2. Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assiut 71524, Egypt;3. School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, Shandong, China;1. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China;2. Bamboo Engineering and Technology Research Center, State Forestry and Grassland Administration, Nanjing 210037, China;3. Dareglobal Technologies Group Co., Ltd., Danyang 212310, China;4. Fujian Jingmingsheng Forestry Technology Co., Ltd., Sanming, 366035, China;1. Institute of Macromolecular Chemistry AS CR, Heyrovsky Sq. 1888/2, 162 06, Prague 6, Czech Republic;2. Department of Nuclear Chemistry, Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University, Břehová 7, 115 19, Prague 1, Czech Republic;3. Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hněvotínská 5, 779 00, Olomouc, Czech Republic;1. Chemistry Department, Faculty of Science, Sohag University, P.O. Box. 82524 Sohag, Egypt;2. Chemistry Department, Faculty of Science, Assiut University, P.O. Box 71516 Assiut, Egypt |
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| Abstract: | To obtain information on the interactions between CETP and HDL3 lipoproteins, we have studied (by surface tension measurements) the adsorption of the CETP at the air–water interface and at the interface between the water and monolayers formed by spreading of lipids extracted from HDL3. We have compared the interfacial behavior of CETP and ApoA-1 (the constitutive protein of HDL3); and the influence of monolayers composition and pressure on the kinetics of the CETP adsorption. The results obtained show that CETP was more expanded than the ApoA-1 which adsorbed more strongly at the air–water interface. CETP adsorbs more and quickly at the lipid interface that at the air–interface, specially for 20% fraction of cholesterol in the monolayer. Our results show that the adsorption of the CETP at the HDL3 surface lipids are strongly dependent of the composition of the monolayer and that the exclusion pressure of CETP varied from 31 to 33.7 mN m−1 with the addition of cholesterol. Finally, the kinetics of the adsorption at water–lipid interface exhibited two steps (quick increase followed by slow decrease of the excess surface pressure) which should indicate a penetration into monolayer followed by a partial desorption of phospholipids with or without cholesterol corresponding to a proteolipid association. |
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