High-performance cascade nanoreactor based on halloysite nanotubes-integrated enzyme-nanozyme microsystem

Various enzymatic reactions or enzymatic cascade reactions occur efficiently in biological microsystems due to space constraints or orderly transfer of intermediate products. Inspired by this, the horseradish peroxidase (HRP)-like nanozyme (Fe-aminoclay) was in situ synthesized on the surface of alkali-activated halloysite nanotubes and the natural enzyme (glucose oxidase, GOx) was immobilized on it to construct a high-efficiency GOx-FeAC@AHNTs cascade nanoreactor. In which, FeAC@AHNTs can not only be used as a carrier for immobilized enzymes, but also help its catalytic activity to cooperate with glucose oxidase in a cascade reaction. The microcompartments and substrate channel effect of this enzyme-nanozyme microsystem exhibit a superior catalytic performance than that of natural enzyme system, and exhibits excellent long-term stability and recyclability. Subsequently, the GOx-FeAC@AHNTs cascade nanoreactor was employed as a glucose colorimetric platform, which displayed a low detection limit (0.47 µmol/L) in glucose detection. This enzyme-nanoenzyme nanoreactor provides a simple and effective example for constructing a multi-enzyme system with limited space, and lays the foundation for subsequent research in the fields of biological analysis and catalysis.