Field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines |
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Authors: | Apichai Santalad Rodjana Burakham Supalax Srijaranai Kate Grudpan |
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Institution: | aDepartment of Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand;bDepartment of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand |
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Abstract: | In-capillary derivatization and field-amplified sample injection (FASI) coupled to capillary zone electrophoresis (CZE) was evaluated for the analysis of metals (Co(II), Cu(II), Ni(II), and Fe(II)) using 2-(5-Nitro-2-Pyridylazo)-5-(N-Propyl-N-Sulfopropylamino)Phenol (Nitro-PAPS) as the derivatizing agent. For FASI, the optimum conditions were water as sample solvent, 1 s hydrodynamic injection (0.1 psi) of a water plug, 5 s of electrokinetic introduction (10 kV) of the sample. The in-capillary derivatization was successfully achieved with zone-passing strategy in order tandem injection of Nitro-PAPS reagent (0.5 psi, 7 s), a small water plug (0.1 psi, 1 s), and metal ion introduction (10 kV, 5 s). The solution of 45 mmol L− 1 borate pH 9.7 and 1.0 × 10− 5 mol L− 1 Nitro-PAPS containing 20% acetonitrile was used as the running buffer. The limit of detection obtained by the proposed method was lower than those from pre-capillary derivatization about 3–28 times. The recovery of the method was comparable to pre-capillary derivatization method. In-capillary derivatization-FASI-CZE was applied to analysis of metals in wine samples. The results were compared with those obtained by CZE with pre-capillary derivatization method and atomic absorption spectrometry (AAS). |
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Keywords: | Capillary zone electrophoresis Field-amplified sample injection In-capillary derivatization Metal ions Nitro-PAPS Wines |
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