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| 谷胱甘肽包被的CdSe/CdS量子点的直接水相制备及其对人血淋巴细胞的标记成像 | |
| 引用本文： | 董微,葛欣,王宣怡,徐淑坤.谷胱甘肽包被的CdSe/CdS量子点的直接水相制备及其对人血淋巴细胞的标记成像[J].光谱学与光谱分析,2010,30(1). |
| 作者姓名： | 董微 葛欣 王宣怡 徐淑坤 |
| 作者单位： | 1. 东北大学化学系,辽宁沈阳110004;沈阳医学院化学系,辽宁沈阳,110034 2. 沈阳医学院化学系,辽宁沈阳,110034 3. 东北大学化学系,辽宁沈阳,110004 |
| 基金项目： | 国家自然科学基金项目(20675011);;辽宁省教育厅基金项目(05L441);;辽宁省自然科学基金项目(20072063)资助 |
| 摘 要： | 首次用谷胱甘肽(GSH)作为稳定剂,在水溶液中制备了稳定地发射绿色荧光和橙色荧光的两种CdSe/CdS核/壳结构的纳米量子点。用紫外-可见分光光度和荧光光谱方法研究了CdSe/CdS量子点的发光特性。透射电镜(TEM)结果表明CdSe/CdS量子点近似球形,在水中分散性良好,比CdSe量子点具有更优异的发光特性,发射光谱和吸收光谱都有红移现象。将CdSe/CdS量子点与鼠抗人CD3抗体连接,制备了水溶性CdSe/CdS-CD3复合物探针,对人血淋巴细胞进行标记和成像。结果表明用该探针对人血淋巴细胞成像清晰,其荧光在30 min的连续蓝光激发下无明显衰退,而FITC荧光在20 min内基本完全猝灭。 |
| 关 键 词： | 谷胱甘肽 CdSe/CdS量子点 免疫荧光标记 人血淋巴细胞 |
Preparation of GSH Capped CdSe/CdS Core-Shell QDs and Labeling of Human T-Lymphocyte |
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| DONG Wei,GE Xin,WANG Xuan-yi,XU Shu-kun.Preparation of GSH Capped CdSe/CdS Core-Shell QDs and Labeling of Human T-Lymphocyte[J].Spectroscopy and Spectral Analysis,2010,30(1). | |
| Authors: | DONG Wei GE Xin WANG Xuan-yi XU Shu-kun |
| Abstract: | Two kinds of L-glutathione capped highly fluorescent CdSe/CdS core-shell quantum dots(QDs)emitting green and orange fluorescence at 350 nm excitation were firstly prepared by an aqueous approach and used as fluorescent labels,to link mouse anti-human CD3 which was expressed on human T-lymphocyte.UV-Vis absorption and fluorescence emission spectra of the as-prepared CdSe/CdS core-shell QDs were studied.Compared with the CdSe QDs,a remarkable enhancement in the emission intensity and a red shift of emission w... |
| Keywords: | Aqueous CdSe/CdS core-shell QDs L-glutathione Immunolabeling Human T-lymphocyte |
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