UPLC-PDA Analysis for Simultaneous Quantification of Four Active Compounds in Crude and Processed Rhizome of Polygonum multiflorum Thunb |
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Authors: | Lifeng Han Bin Wu Guixiang Pan Yuefei Wang Xinbo Song Xiumei Gao |
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Institution: | 1. Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, People’s Republic of China 2. Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, 310058, Hangzhou, People’s Republic of China
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Abstract: | A novel, rapid and specific ultra performance liquid chromatography-photo diode array detection method was developed for the simultaneous determination of 2,3,5,4′-tetrahydroxystilbene-2-O-β-d-glucoside (TSG), emodin-8-O-β-d-glucoside (EMG), emodin (EM) and physcion (PS). The chromatographic separation was performed on an Acquity BEH C18 column (100 × 2.1 mm i.d., 1.7 μm). The mobile phase was a mixture of 0.3% acetic acid–water and 0.3% acetic acid–acetonitrile employing gradient elution at the flow rate of 0.4 mL min?1. The four compounds behaved linearly in the concentration range between 60.80–3040.00 μg mL?1 (TSG), 0.50–25.00 μg mL?1 (EMG), 2.16–108.00 μg mL?1 (EM) and 1.56–78.00 μg mL?1 (PS), respectively with correlation coefficients >0.999. The precision of the method were below 5% RSD. Recoveries of the four compounds ranged from 95.71 to 102.97%, with RSD values less than 2%. |
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