| 大孔树脂-高速逆流色谱分离纯化薇甘菊中的黄酮类化合物 |
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| 引用本文： | 耿姗,王娟强,席兴军,初侨,董跟来,马晓萌,张惠文,魏芸.大孔树脂-高速逆流色谱分离纯化薇甘菊中的黄酮类化合物[J].色谱,2017,35(3):302-307. |
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| 作者姓名： | 耿姗 王娟强 席兴军 初侨 董跟来 马晓萌 张惠文 魏芸 |
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| 作者单位： | 1. 北京化工大学化工资源有效利用国家重点实验室, 北京 100029;
2. 中国标准化研究院, 北京 100191;
3. 北京化工大学理学院, 北京 100029 |
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| 基金项目： | National Natural Science Foundation of China (No. 21075007); Program for New Century Excellent Talents in University (No. NCET-11-0563); Beijing Nova Program Interdisciplinary Cooperation Project (No. Z161100004916045). |
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| 摘 要： | 该文建立了大孔树脂-高速逆流色谱分离薇甘菊中黄酮类物质的方法。分离条件为:采用大孔树脂AB-8，洗脱液为50%（v/v）乙醇水溶液，高速逆流色谱溶剂体系为正丁醇-乙酸-水（4：1：5，v/v）。从薇甘菊中分离到4种黄酮类物质:槲皮素-3-O-芸香糖苷（纯度90.2%）、山奈酚-3-O-芸香糖苷（纯度98.55%）、木犀草苷（纯度98.33%）和紫云英苷（纯度99.23%）。建立的大孔树脂-高速逆流色谱方法简单、高效，可扩展应用于从其他植物中分离黄酮类物质。
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| 关 键 词： | 高速逆流色谱 大孔树脂 分离 黄酮类物质 薇甘菊 |
| 收稿时间： | 2016-10-12 |
Separation and purification of flavonoids from Mikania micrantha by macroporous resin combined with high speed countercurrent chromatography |
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| GENG Shan,WANG Juanqiang,XI Xingjun,CHU Qiao,DONG Genlai,MA Xiaomeng,ZHANG Huiwen,WEI Yun.Separation and purification of flavonoids from Mikania micrantha by macroporous resin combined with high speed countercurrent chromatography[J].Chinese Journal of Chromatography,2017,35(3):302-307. |
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| Authors: | GENG Shan WANG Juanqiang XI Xingjun CHU Qiao DONG Genlai MA Xiaomeng ZHANG Huiwen WEI Yun |
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| Institution: | 1. State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China;
2. China National Institute of Standardization, Beijing 100191, China;
3. School of Sciences, Beijing University of Chemical Technology, Beijing 100029, China |
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| Abstract: | The present study established a method for the separation and purification of four flavonoids quercetin-3-O-rutinoside, kaempferol-3-O-rutinoside, luteoloside and astragalin from Mikania micrantha by using macroporous resin combined with high speed countercurrent chromatography (HSCCC). Macroporous resin was AB-8 and the eluent was 50% (v/v) ethanol. A two-phase solvent system composed of n-butanol/acetic acid/water (4:1:5, v/v) was used in HSCCC. Quercetin-3-O-rutinoside, kaempferol-3-O-rutinoside, luteoloside and astragalin with the purity of 90.12%, 98.55%, 98.33% and 99.23%, respectively, were successfully separated from Mikania micrantha by HSCCC using the lower phase of this solvent system as mobile phase in one run. This study pave a simple and efficient method that could be used in the following separation of flavonoids from invasive plants. |
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| Keywords: | high speed countercurrent chromatography (HSCCC) macroporous resin separation flavonoids Mikania micrantha (M micrantha) |
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