Simultaneous production of citric acid and erythritol from crude glycerol by Yarrowia lipolytica Wratislavia K1 |
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Authors: | Waldemar Rymowicz Anita Rywińska Witold Gładkowski |
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Institution: | (1) Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, 503 75 Wrocław, Poland;(2) Department of Chemistry, Faculty of Food Science, Wrocław University of Environmental and Life Sciences, 503 75 Wrocław, Poland |
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Abstract: | This study shows a possible microbial process for utilization of crude glycerol generated by the biodiesel industry for citric
acid and erythritol production. Simultaneous production of citric acid and erythritol under nitrogen-limited conditions with
glycerol as the carbon source was achieved with an acetate negative mutant of Y. lipolytica Wratislavia K1 in fed-batch cultivations. The effect of the initial glycerol concentration (from 30–180 g dm−3) on the citrate and erythritol production was investigated. As a result of the experiments, maximum citric acid production
(110 g dm−3) and a very high amount of erythritol (81 g dm−3) were determined after 168 h of fed-batch cultivation with the initial glycerol concentration of 150 g dm−3 and the total glycerol concentration of 250 g dm−3. In addition, the citric acid to isocitric acid ratio of the products from this strain was 35.5:1.
Presented at the 34th International Conference of the Slovak Society of Chemical Engineering, Tatranské Matliare, 21–25 May
2007. |
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Keywords: | crude glycerol citric acid erythritol Yarrowia lipolytica fed-batch mode |
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