Comparison of high-speed counter-current chromatography instruments for the separation of the extracts of the seeds of Oroxylum indicum |
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Authors: | Chen L J Song H Lan X Q Games D E Sutherland I A |
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Institution: | State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Chengdu, Sichuan 610041, China. Iijuanl7@hotmail.com |
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Abstract: | Analytical Milli high-speed counter-current chromatography (HSCCC) was used for the selection and optimization of the two-phase solvent system to separate flavonoids from the extracts of the seeds of Oroxylum indicum. The optimum solvent system obtained from Milli-CCC was also the best solvent system for preparative HSCCC and led to the successful separation of two crude flavonoids from the seeds of O. indicum by Lab/Prep (laboratory preparative) HSCCC using different sized coils. Four flavonoids were isolated by preparative HSCCC: baicalein-7-O-diglucoside (25.0 mg, 92% purity), baicalein-7-o-glucoside (50.4 mg; 95% purity), baicalein (75 mg; purity 98%) and chrysin (100 mg; purity 98%). |
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