Fabrication of a novel impedance cell sensor based on the polystyrene/polyaniline/Au nanocomposite

Gold nanoparticles (AuNPs) were assembled on the surface of polystyrene (PS) and polyaniline (PANI) core-shell nanocomposite (PS@PANI) for the immobilization of HL-60 leukemia cells to fabricate a cell electrochemical sensor. The immobilized cells exhibited irreversible voltammetric response and increased the electron transfer resistance with a good correlation to the logarithmic value of concentration ranging from 1.6 × 10³ to 1.6 × 10⁸ cells mL⁻¹ with a limit of detection of 7.3 × 10² cells mL⁻¹ at 10σ. This biosensor was simple, low cost and disposable, which implied that the PS@PANI/Au composites can regard as the potential applications for clinical applications.