Synthesis of glutamate by reductive amination of 2-oxoglutarate with the combination of hydrogenase and glutamate dehydrogenase |
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| Authors: | Fumihiko Hasumi Yasumitsu Miyamoto Ichiro Okura |
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| Institution: | (1) Department of Chemistry and Biochemistry, Numazu College of Technology, Shizuoka, Japan;(2) Department of Bioengineering, Tokyo Institute of Technology, Nagatsuta, Midori-ku, 226 Yokohama, Japan |
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| Abstract: | Glutamate synthesis by reductive amination of 2-oxoglutarate was performed by the combination of NADH regeneration system
and glutamate dehydrogenase (GluDH). The conversion of 2-oxoglutamate to glutamate was 98% after 3 h, and the turnover number
of NAD+was 17. |
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| Keywords: | Hydrogenase glutamate dehydrogenase NAD+ hydrogenation |
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