| 兽疫链球菌变异株产生的透明质酸的纯化及表征 |
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| 引用本文： | 洪水声,陈佳,滕利荣,孟庆繁,张建国,刘兰英.兽疫链球菌变异株产生的透明质酸的纯化及表征[J].高等学校化学学报,2004,25(5):853-857. |
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| 作者姓名： | 洪水声 陈佳 滕利荣 孟庆繁 张建国 刘兰英 |
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| 作者单位： | 1. 吉林大学生命科学学院, 长春 130023;
2. 中国科学院长春应用化学研究所电分析化学国家重点实验室, 长春 130022 |
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| 基金项目： | 国家高等教育科学“十五”规划项目 (批准号 :2 0 0 2 ,2 0 -5 8-4 7)，吉林省教育科学十五规划重点项目(批准号 :2 0 0 1-11-0 15 3 3 8)资助 |
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| 摘 要： | 用N-甲基-N′-硝基-N-亚硝基胍(NTG)对兽疫链球菌进行诱变,获得高产菌株.经过对该菌株的发酵培养,将产生的多糖经Savage法、季铵复合物沉淀法、DEAE-纤维素(DE52)离子交换层析法及SephadexG-75凝胶过滤法分离纯化.纯化的多糖结构经化学组成分析、核磁共振波谱、红外光谱及圆二色谱鉴定,证明了诱变得到的高产菌株(StreptococcuszooepidemicusJ18)再经发酵,得到的多糖为透明质酸.通过刚果红实验证明了透明质酸的构象为单股螺旋结构,其平均分子量约为1.16×106.
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| 关 键 词： | 兽疫链球菌变异株 透明质酸 分离纯化 表征 |
| 文章编号： | 0251-0790(2004)05-0853-05 |
| 收稿时间： | 2003-06-05 |
Purification and Structure Characterization of the Hyaluronic Acid Produced by a Streptococcus Zooepidemicus Mutation Strain |
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| HONG Shui-Sheng ,CHEN Jia ,TENG Li-Rong ,MENG Qing-Fan ,ZHANG Jian-Guo ,LIU Lan-Ying.Purification and Structure Characterization of the Hyaluronic Acid Produced by a Streptococcus Zooepidemicus Mutation Strain[J].Chemical Research In Chinese Universities,2004,25(5):853-857. |
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| Authors: | HONG Shui-Sheng CHEN Jia TENG Li-Rong MENG Qing-Fan ZHANG Jian-Guo LIU Lan-Ying |
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| Institution: | 1. College of Life Science, Jilin University, Changchun 130023;
2. State Key Laboratory of Electroanalytical Chemistry, Chinese Acadamy of Sciences, Changchun Institute of Applied Chemistry, Changchun 130022, China |
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| Abstract: | A mutated strain of Streptococcus zooepidemicus capable of producing the polysaccharide in a high yield was obtained after being mutated bythe chemical agent N-methyl-N′-nitro-N- nitrosoguanidine(NTG). Polysaccharide produced bythe mutated strain of Streptococcus zooepidemicus was purified bythe procedure including Savage method, quaternary ammonium compound precipitation, DEAE-cellulose(DE52) column chromatograph and Sephadex G-75 gel filtration. The purified polysaccharide was proved to be hyaluronic acid(HA) by means of chemical composition analysis, 13C NMR, Infrared spectra, and circular dichroism. The single helix conformation of the purified HA was studied by Congo red experiment. The molecular weight of the HA was about 1.16×106, which was measured by viscosity method. |
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| Keywords: | Streptococcus zooepidemicus Hyaluronic acid Separation and purification Characterization |
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