Real-time imaging of cell-surface proteins with antibody-based fluorogenic probes |
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| Authors: | Wenchao Wang Ying Zhang Hong Zhao Xinlei Zhuang Haoting Wang Kaifeng He Wanting Xu Yu Kang Shuqing Chen Su Zeng Linghui Qian |
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| Institution: | Institute of Drug Metabolism and Pharmaceutical Analysis, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Sciences, Cancer Center, Zhejiang University, Hangzhou 310058 China.; Hangzhou Institute of Innovative Medicine, Zhejiang University, Hangzhou 310018 China |
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| Abstract: | Cell-surface proteins, working as key agents in various diseases, are the targets for around 66% of approved human drugs. A general strategy to selectively detect these proteins in a real-time manner is expected to facilitate the development of new drugs and medical diagnoses. Although brilliant successes were attained using small-molecule probes, they could cover a narrow range of targets due to the lack of suitable ligands and some of them suffer from selectivity issues. We report herein an antibody-based fluorogenic probe prepared via a two-step chemical modification under physiological conditions, to fulfill the selective recognition and wash-free imaging of membrane proteins, establishing a modular strategy with broad implications for biochemical research and for therapeutics.A modular strategy to convert commercially available antibodies into fluorogenic probes has been developed, enabling selective recognition and wash-free imaging of endogenous membrane proteins. |
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