Simple and sensitive fluorescence sensor for detection of potassium ion in the presence of high concentration of sodium ion using berberine–G-quadruplex complex as sensing element |
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Authors: | Yanyan Liu Baoxin Li Dongmei Cheng Xiaoyan Duan |
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Institution: | aKey Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, People's Republic of China |
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Abstract: | In this work, a novel potassium ion (K+) sensor is presented using berberine–G-quadruplex complex as a fluorescent probe. This sensor is based on the K+that can induce the G-rich DNA to form G-quadruplex conformation. The G-quadruplex can bind berberine to form berberine–G-quadruplex complex, resulting in remarkable enhancement of fluorescence emission of the berberine–G-quadruplex system. In the presence of 800 mM sodium ion (Na+), the fluorescence of the berberine–G-quadruplex complex increased linearly with increasing K+ concentration in the range of 0.005–1.0 mM. The turn-on fluorescent assay is simple, inexpensive, and highly sensitive. We observed that Na+ in 10,000-fold molar excess does not interfere. The molecular mechanisms which produce enhanced fluorescence of berberine were discussed. |
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Keywords: | Potassium ion G-quadruplex Berberine Fluorescence sensor |
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