采用含原位聚合阴离子交换整体柱的微芯片分离氨基酸

采用原位聚合法,制备了以2-甲基丙烯酰氧乙基三甲基氯化铵(META)为功能单体的强碱性季铵盐离子交换型微整体柱,构建了带微整体柱的复合式微流控芯片;以氨基酸-H2O2-Lum inol化学发光体系为样品对象,根据氨基酸等电点的差异,在原位聚合微整体柱上进行分离实验。进行了苯丙氨酸-白氨酸、苯丙氨酸-组氨酸、苯丙氨酸-精氨酸3组氨基酸混合体系的分离,获得了很好的分离结果。优化并讨论了影响氨基酸分离效果的多种因素,如缓冲液pH值、洗脱液pH值和洗脱液流速等。在优化条件下,苯丙氨酸-精氨酸的分离度达到1.6,结果显示出整体柱与微流控体系相结合的可行性。

Separation of Amino Acid by In-situ Polymerized Anion Exchange Micro- monolithic Column on Micro-fluidic Chip

Alkali quaternary ammonium anion exchange micro-monolithic column based on monomer of(2-(methacryloyloxy)ethyl) trimethylammonium chloride(META) was polymerized in-situ.The micro-monolithic column was combined with micro-fluidic chip.The amino acids-H_2O_2-luminol chemiluminescence system was taken as samples on the combinatorial micro-fluidic chip.Due to the difference of isoelectric point(pI)of amino acids,separation experiments were performed on the micro-monolithic column.The separation of amino acid samples including phenylalanine-leucine,phenylalanine-histidine and phenylalanine-arginine,was(carried) out on the set-up microchip.The results showed that complete separation of samples was realized.(Several) factors effected on amino acids separation,such as buffer pH,elution pH and flow rate were(optimized).Under the optimized conditions,the resolution of phenylalanine-arginine was 1.6.The results also demonstrated(feasibility) of integrating the micro-monolithic column on micro-fluidic chip.