Determination of Tetracycline at a UV‐Irradiated DNA Film Modified Glassy Carbon Electrode |
| |
Authors: | Mohammad Bagher Gholivand Hamzeh Khani |
| |
Institution: | 1. Department of Analytical Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran tel: +98?831?4274557;2. Department of Education, Ilam, Iran |
| |
Abstract: | In this study, interaction of tetracycline (TC) and DNA in the Britton? Robinson buffer solution (BR) was studied by cyclic voltammetry. The experimental results reveal that TC can bind strongly to DNA and the association constant and binding number between TC and DNA was obtained. Then DNA was immobilized on a glassy carbon electrode by UV‐irradiation. Through this process, water‐soluble DNA was converted into insoluble materials, and a stable DNA film was formed on the electrode. The electrochemical oxidation behavior of TC was studied at UV‐irradiated DNA film modified glassy carbon electrode (UV‐DNA‐GCE). The response of modified electrode was optimized with respect to pH, accumulation time, ionic strength, drug concentration and other variables. TC at the surface of modified electrode showed a linear dynamic range of 0.30–90.00 µM and a detection limit of 0.27 µM. To demonstrate the applicability of the modified electrode, it was used for the analysis of real samples such as pharmaceutical formulations and milk. |
| |
Keywords: | Cyclic voltammetry Differential pulse voltammetry DNA‐modified electrode Tetracycline‐DNA interaction |
|
|